Microbial Proteomics

The pre-course "Microbial Proteomics" will be held at Porto conte Ricerche, Alghero (Italy) from June 13th to 14th, 2017.

Background. Numerous progresses have recently been made in the proteomic analysis of single microbial isolates, enabling the detailed characterization of the different proteomes expressed by a bacterial genome in various conditions. In parallel, this has also opened the invaluable possibility of gathering information on all the microbial proteomes expressed by a microbial community: the metaproteome.

Aims. This “Microbial Proteomics” course is intended for graduate students and young researchers who wish to improve their analytical capabilities in the field of microbiology by implementing proteomic technologies in their study areas, but also to those that already have experience in proteomics but would like to gain a better understanding of the practical aspects and analytical issues involved in the application of proteomic technologies to microorganisms and microbial systems.

The pre-corse is expected to enable young graduate students a more profitable participation to the associate "2nd International Metaproteomics Symposium" that will be held from June 14th to 16th, 2017.

Corse venue

Porto Conte Ricerche is a research centre of the Science and Technology Park of Sardinia.The technological laboratories at Porto Conte Ricerche enable the integrated application of methodologies and experimental pipelines with a focus on industrial research, and are devoted to the development of knowledge, processes, products and services with applications in biotechnology (biomedicine, agroindustry, environment) and in food technology (quality, safety and sustainability in food distribution, consumers’ health, trademarks and labels).Since 2009, Porto Conte Ricerche has been actively involved in research projects aimed to the development of methods and pipelines for the characterization of microbial proteomes and microbial community proteomes, and in their application for biomarker discovery in human medicine, in veterinary medicine, and in the agrifood sector. A specific research focus has been the investigation of the gut microbiota and of its relationships with the host genetic makeup (and of its connections with gut health and the predisposition to develop autoimmune diseases) as well as with environmental factors (including nutrition). The research activities have covered different aspects of microbial proteomics, from optimization of protein extraction methods, to analysis of tryptic peptides by high resolution mass spectrometry, to the development of dedicated bioinformatic pipelines for data analysis and interpretation.

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Programme

“Microbial Proteomics”: Preliminary ProgramIntroduction to Microbial ProteomicsThree theoretical and practical sessions covering the following topics:Opportunities, relevance and application of proteomic technologies to the characterization of microorganisms;Methods for analyzing the proteome and the sub-proteome of cultured microorganisms, from protein extraction to sample characterization;Methods for analyzing the proteome of microorganisms extracted from naturally or experimentally infected tissues/organs, from protein extraction to sample characterization;Preparation of protein extracts for high resolution mass spectrometry analysis (shotgun proteomics);Analysis of peptide mixtures by high resolution mass spectrometry (shotgun proteomics);Identification and taxonomical-functional annotation of peptides/proteins with bioinformatic tools (generation of sequence databases, database search, taxonomic annotation, functional annotation).Number of participants: MAX 20Lecturers: Sergio Uzzau, Maria Filippa Addis, Daniela Pagnozzi and Alessandro Tanca.

Registration

Registration fees include: theoretical and practical sessions of the Microbial Proteomics course, course material, lunches and coffee breaks (June 13th and 14th, 2017)The registration fee is € 150.When registering also to the “2nd International Metaproteomics Symposium” the registration fee is reduced to € 100.To register for the course please send an e-mail to [email protected] indicating as subject “Microbial Proteomics registration”. Please also include a short CV and a description of your interest and motivation to participate. After confirmation of acceptance by e-mail, the fee can be paid by bank transfer.Payments should be made to:• Recipient: Porto Conte Ricerche• Bank Name: Banco di Sardegna• IBAN: IT89R0101517200000070427713• BIC: BPMOIT22 XXX• Reference: MPC2017 ‒ Surname NameThe registration will be confirmed by e-mail after receiving the bank transfer.DEADLINE: April 3rd, 2017