Programme

In accordance with the HUPO/EuPA guidelines, we aim to:

• Provide a theoretical basis for understanding gel-based proteomics
• Illustrate how the techniques are being applied in modern proteomics studies
• Help students to design their own experiments
• Provide practical instruction in laboratory techniques
• Provide extensive tutorial/discussion sessions

Theoretical lectures
• Basic electrophoresis terms and theory
• Experimental design and sample preparation
• Multi-dimensional electrophoretic separations (2D-PAGE / 2D-DIGE)
• Gel image analysis
• Mass Spectrometry and database searching

Practical classes
• Sample preparation: protein extraction and labelling
• Evaluating and optimising separations
• Making 2D separations (2D-PAGE / 2D-DIGE)
• Image analysis
• Spot cutting, digestion, and protein identification by MS